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Effect of osmolality on basal and angiotensin II-stimulated aldosterone secretion in-vitro by bovine adrenal glomerulosa cells. The sodium and potassium concentrations were measured by flame photometry, osmolality was measured by freezing point depression, and the aldosterone level was measured in duplicate directly, using a commercial RIA Kit Diagnostic products, Los Angeles CA. National Center for Biotechnology Information , U. Glomerulosa cells were incubated under one of the following two media: The effect of different Na concentrations in the incubation media on basal and All-stimulated aldosterone secretion is shown in Figure 1.

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Effects of lithium on angiotensin-stimulated phosphatidylinositol turnover and aldosterone production in adrenal glomerulosa cells: Most users should sign in with their email address.

The mechanism responsible for the effects of Na ion on aldosterone secretion was not addressed in this study. With these new introductions, AMD now offers a top-to-bottom line-up of professional-grade computing solutions for experiences that range from premium content creation to advanced multitasking and office productivity. A p-value below 0.

WCH of warrants to purchase 75 million shares of its common stock, if and when exercised, will dilute the ownership interests of its existing stockholders, and the conversion of the 2. The osmolality of this solution was between and mOsm. A crusher is a machine designed to reduce large rocks into smaller rocks, gravel, or rock dust.

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Seong Yeon KimM. The osmolality of this solution was between and mOsm. Solutions Basalt stone processing plant Coal beneficiation plant Feldspar stone processing plant Kaolin processing plant Sand Making Plant. The increment in aldosterone above basal levels by All stimulation was impaired at 50mM-Na medium and reached a maximum at 93mM-Na medium.

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Performance listed in order of Pentium G vs. The effect on aldosterone secretion of total Cl ion removal in the incubation medium was also gs5093. Extracellular choline is a constituent of acetylcholine and may act as an acetylcholine analogue which has been found to stimulate aldosterone secretion by isolated bovine glomerulosa cells through action similar to that of Fs5093 on glomerulosa cells.

Vs509 do not currently have access to this article. Oxford University Press is a department of the University of Oxford. These results suggest that Cl ion may play a minor role in aldosterone secretion. Go to this page to see a list of supporting browsers. Close mobile search navigation Article navigation. Physiology and Pathophysiology p.

Contact us For product information and price, chat with sales agent. It is well recognized that Na ion is the main extracellular cation, and in addition to its role of maintaining extracellular osmolality, it plays many roles in cellular functions. In contrast, Schiffrin et al 9 and Ball et al, 10 demonstrated that low extracellular Na concentrations inhibited aldosterone secretory response to All. After preincubating the cells as in the previous experiment, the final cell suspension was divided into two aliquots and each aliquot was washed twice with each medium.

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Clin, and Exper-Theory and Practice A8 3: Glomerulosa cells were incubated under one of the following media of different Na concentrations in which NaCl was replaced iso-osmotically by choline Cl or N-methylglucamine Cl: The tubes were stoppered and incubated at 37C in a Dubnoff metabolic shaking incubator for 90 minutes.

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Biochim Biophys Res Commun Electrical properties of isolated rat adrenal glomerulosa and fasciculata cells. AMD websiteblogFacebook and Vs509 pages. These media were prepared by mixing the appropriate portion of mM-Na solution with that of mM choline Cl or N-methylglucamine Cl solution O-Na solution.

Cellular responses to extracellular osmolality. The effect of different Na concentrations in the incubation media on basal and All-stimulated aldosterone secretion is shown in Figure 1.

Analytic Methods The sodium and potassium concentrations were measured by flame photometry, osmolality was measured by freezing point depression, and the aldosterone level was measured in duplicate directly, using a commercial RIA Kit Diagnostic products, Los Vs0593 CA.

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